Dr. Hasthi Ram
Plant Molecular Biology and Biotechnology, Functional Genomics, Micronutrient partitioning in seeds
Post-doctoral Fellow at European Molecular Biology Laboratory (EMBL), Heidelberg, Germany (Apr 2012 - Dec 2016)
Using FACS associated RNA-Seq and Live-Imaging, we identified cell-type specific targets of transcription factors associated with organ development at Shoot Apical Meristem (SAM) in Arabidopsis thaliana.
Ph.D. at National Institute of Plant Genome Research (NIPGR), New Delhi, India (July 2007 – Mar 2012)
Using various molecular genetics and genomics tools, we established functional interrelationship among three bZIP transcription factors for light mediated seedling developemnt in Arabidopsis thaliana.
- Developed a genomic resource of direct targets of bZIP transcription factor GBF1 in Arabidopsis thaliana.
- In plant system first time our work has shown the importence of bZIP heterodimerization for their in-vivo genome-wide binding.
- Developed and shown usefullness of multi-colour FACS appraoch in plants.
- Developed gene expression atlas of diferent doamins of Arabidopsis Shoot Apical Meristem.
- Developed method to collect different cell-types of developing rice grains using Laser Capture Microdissection (LCM).
- Developed gene expression atlas of differnet cell-types of nutrient filling rice grains.
During post-harvest processing (polishing/milling) of brown rice, bran layer consisting of aleurone layer, pericarp and embryo is removed to produce white rice. Bran is nutritionally superior as it has major reservoirs of various minerals, vitamins, essential mineral oils and other bioactive compounds, whereas white rice portion is nutritionally inferior as it mainly contains starchy endosperm. Irony is that nutritionally inferior white rice is a major staple food for almost half of the country, whereas almost 2/3rd of nutrient rich bran fraction is often get wasted as inedible due to instability of the bran. Thus both the products of the rice post-harvest process, the white rice and the bran, have their own inherent problems. To address this, we aim to increase nutritional value of the endosperm and increase the stability of bran through biotechnological approaches. Towards this end, using high-resolution, cell-type specific transcriptomics approach we have prepared a gene expression atlas of developing rice grain and identified cell-type specific genes. From this very highly-resolved gene expression dataset, we have identified certain micronutrient transporter genes, and studying their role to increase the nutritional value of the endosperm. For increasing the stability of bran we are looking at role of various hydrolytic enzymes such as GDSL lipases, estrases, etc., which have very cell-type specific expression in our transcriptomics dataset. We are using various molecular biology tools such as cell-type specific transcriptomics, in-situ hybridisation, CRISPR-Cas9 system, expression in heterologous system and plant tissue culture approaches for our studies.
Ram H, Sahadevan S, Gale N, Caggiano MP, Yu X, Ohno C, et al. (2020) An integrated analysis of cell-type specific gene expression reveals genes regulated by REVOLUTA and KANADI1 in the Arabidopsis shoot apical meristem. PLoS Genet 16(4): e1008661. https://doi.org/10.1371/journal.pgen.1008661
Ram H*, Gandass N, Sharma A, Singh A, Sonah H, Deshmukh R, Pandey AK, Sharma TR* (2019) Spatio-temporal distribution of micronutrients in rice grains and its regulation. Critical review in Biotechnology.DOI:10.1080/07388551.2020.1742647
Merelo P, Ram H, Pia Caggiano M, Ohno C, Ott F, Straub D, Graeff M, Cho SK, Yang SW, Wenkel S, et al. (2016) Regulation of MIR165/166 by Class II and Class III homeodomain leucine zipper proteins establishes leaf polarity. Proceedings of the National Academy of Sciences (PNAS), USA, 2016, 113 (42), 11973-11978. https://doi.org/10.1073/pnas.1516110113 (IF=9.5)
Ram H, Priya P, Jain M, Chattopadhyay S (2014) Genome-wide DNA binding of GBF1 is modulated by its heterodimerizing protein partners, HY5 and HYH. Molecular Plant. 7(2): 448-451. DOI: http://dx.doi.org/10.1093/mp/sst143 (IF=10.2)
Ram H#, Singh A#, Abbas N, Chattopadhyay S (2012) Molecular interactions of GBF1 with HY5 and HYH proteins during light-mediated seedling development in Arabidopsis thaliana. J Biol Chem. 287(31): 25995-6009 (#Equal contribution). DOI:10.1074/jbc.M111.333906 (IF=4.0)View All Publication
Ram H, Jain M, Singh A, Chattopadhyay S (2016) Functional Relationship of GBF1 with HY5 and HYH in Genome-Wide Gene Expression in Arabidopsis. Plant Molecular Biology Reporter. 34(1): 211-220. DOI: 10.1007/s11105-015-0910-x (IF=2.0)
Ram H*, Kaur A, Gandass N, Singh S, Sonah H, Deshmukh R, Sharma TR (2019). Molecular characterization and expression dynamics of MTP genes under various spatio-temporal stages and metal stress conditions in rice. PLoS One https://doi.org/10.1371/journal.pone.0217360 14(5): e0217360. (IF=2.8)
Ram H, Chattopadhyay S (2013) Molecular interaction of bZIP domains of GBF1, HY5 and HYH in Arabidopsis seedling development. Plant Signaling & Behavior. 8(1): e22703 DOI: https://doi.org/10.4161/psb.22703 (IF~1.0)
Ram H, Singh BP, Katara J, Kumar P, Jaiswal R, Geetika, Deshmukh R, Sonah H (2019). A Genome-wide Resource of Intron Spanning Primers Compatible for Quantitative PCR and Intron Length Polymorphism in Rice. Indian Journal of Genetics and Plant Breeding. 79(2) (2019). (IF=0.4)
Caggiano MP, Yu X, Bhatia N, Larsson A, Ram H, Ohno CK, et al. (2017) Cell type boundaries organize plant development. eLife. 6. DOI: 10.7554/eLife.27421 (IF=7.6)
Chakraborty M, Gangappa SN, Maurya JP, Sethi V, Srivastava A, Singh A, Dutta S, Gupta N, Sengupta M, Ojha M, Ram H, Chattopadhyay S. Functional Interrelation of MYC2 and HY5 Plays an Important Role in Arabidopsis Seedling Development. Plant Journal, https://doi.org/10.1111/tpj.14381 2018 (IF=5.8)
Giri MK, Banday ZZ, Singh N, Singh V, Ram H, Singh D, Chattopadhyay S, Nandi AK (2017) GBF1 differentially regulates CAT2 and PAD4 transcription to promote pathogen defense in Arabidopsis thaliana. The Plant Journal. 91(5): 802–815 https://doi.org/10.1111/tpj.13608 (IF=5.8)
Gangappa SN, Srivastava AK, Maurya JP, Ram H, Chattopadhyay S (2013) Z-box binding transcription factors (ZBFs): a new class of transcription factors in Arabidopsis seedling development. Molecular Plant. 6(6): 1758-1768 DOI: http://dx.doi.org/10.1093/mp/sst140 (IF=10.2)
Mallappa C, Singh A, Ram H, and Sudip Chattopadhyay (2008) GBF1, a transcription factor of blue light signaling in Arabidopsis, is degraded in the dark by a proteasome-mediated pathway independent of COP1 and SPA1. J Biol Chem. 283: 35772-35782 DOI: 10.1074/jbc.M803437200 (IF=4.0)
Ram H*, Soni P, Salvi P, Gandass N, Sharma A, Kaur A, Sharma TR* (2019). Insertional Mutagenesis Approaches And Their Use in Rice for Functional Genomics. Plants, 8(9), 310; https://doi.org/10.3390/plants8090310
Sharma S, Kaur G, Kumar A, Ram, H, Kaur J and Pandey AK* (2020). Gene expression pattern of vacuolar-iron transporter-like (VTL) genes in hexaploid wheat during metal stress. Plants (MDPI) 9(2), 229. https://doi.org/10.3390/plants9020229
- Awarded NASI-Young Scientist Platinum Jubilee Award by the National Academy of Sciences, India (NASI) (2019).
- Awarded DST-INSPIRE Faculty award by DST, Government of India (2017).
- Awarded Junior and Senior Research Fellowships (JRF and SRF) by CSIR, Government of India (2007).
- Qualified National Eligibility Test (NET) for Lectureship conducted by UGC and CSIR, Government of India (2007).
- Qualified Graduate Aptitude Test in Engineering (GATE) conducted by Ministry of human resource development, Government of India (2007).